【摘要】 以坛紫菜耐高温型品系Z-61F4代叶状体为实验材料,野生型坛紫菜(WT)叶状体为对照,研究了常温(21℃)静水和高温(30℃)静水培养不同天数(0、2、4、6、8、10d)后,坛紫菜藻体中光合色素含量、粗蛋白含量、总氨基酸含量和4种主要呈味游离氨基酸含量等品质指标的变化情况。结果表明,常温静水培养对坛紫菜耐高温型品系的品质没有显著影响,甚至短时间的常温静水培养还有利于提高野生型品系藻体的品质;但高温静水培养会显著降低耐高温型品系和野生型品系的品质,只是耐高温型品系的品质下降速度要慢于野生型。此外,本研究还发现,耐高温型坛紫菜品系还可以通过调节藻体细胞内别藻蓝蛋白(APC)和游离氨基酸的含量来增强自身对高温胁迫的抵抗能力。本研究结果为全面认识坛紫菜的抗逆性机理提供了基础资料,同时也为后续坛紫菜抗逆新品种的选育提供了理论指导。更多还原

【Abstract】 Porphyra haitanensis is an economically important marine crop in southern China.Due to global warming,farming of P.haitanensis was seriously affected by the increasing water temperature.In this study,the gametophytic blades of an F4high temperature tolerance line(Z-61)and wild-type line(WT)were grown at normal temperature(21℃)and high temperature(30℃)under non-aerated condition for 0,2,4,6,8and 10days.Changes in four quality indices were measured,including contents of pigment,crude protein,total amino acids and free amino acids.The results showed that the quality of Z-61was not significantly affected by the normal temperature and non-aeration.The quality of wild-type strain was even improved after a short-term cultivation under normal temperature and non-aeration,but the quality of Z61and wild-type strains were seriously affected by the high temperature and non-aerationstress.Furthermore,the results also indicated that the Z-61could enhance its ability of high temperature tolerance by increasing the content of allophycocyanin(APC)and free amino acids.Our results would be helpful for understanding the mechanism of high temperature tolerance of P.haitanensis.更多还原