【摘要】 目的探讨慢性心房纤颤(房颤)犬心房组织中髓过氧物酶(MPO)的变化及其意义。方法健康杂种犬20条,随机分为房颤组(n=10)和对照组(n=10)。房颤组使用永久起搏器起搏24周,制备房颤犬模型;对照组不置入起搏器。取心房组织标本,分别采用ELISA法、同位素稀释气相色谱质谱法、Masson染色、明胶酶谱法测定MPO含量、3-氯酪氨酸含量、胶原容积分数(CVF)、基质金属蛋白酶-2和9(MMP-2、MMP-9)活性。结果房颤组心房肌组织MPO含量、3-氯酪氨酸含量、CVF、MMP-2和MMP-9活性较对照组显著升高(P<0.05)。MPO含量与CVF显著正相关(P<0.05)。结论 MPO可能是慢性房颤发生和维持的重要介质。更多还原

【Abstract】 Objective To investigate the changes and significance of myeloperoxidase(MPO) in atrial tissues of dogs with chronic atrial fibrillation.Methods Twenty mongrel dogs were randomly divided into atrial fibrillation group(n=10) and control group(n=10).The High frequency pacing was performed in atrial fibrillation group with permanent pacemaker,but not in the control group.Twenty-four weeks after pacing,atrial tissue was taken.The contents of MPO content and 3-chlorotyrosine were measured by ELISA and stable isotope dilution liquid chromatography with on-line tandem mass spectrometry,respectively.The collagen volume fraction(CVF) and activities of matrix metalloproteinase-2 and 9(MMP2,MMP-9) were determined by Masson staining and gelatin zymography,respectively.Results The levels of MPO,3-chlorotyrosine,CVF and activities of MMP-2 and MMP-9 were significantly higher in atrial fibrillation group than those in control group(P<0.05).The MPO content was significantly correlated with CVF(P<0.05).Conclusion MPO is possibly one of important mediators mechanistically involved in the initiation and perpetuation of chronic atrial fibrillation.更多还原