【摘要】 以Cu(Ⅱ)作为中心离子,谷氨酸(Glu)和精氨酸(Arg)作为配体合成了铜离子混配合物[Cu(Glu·Arg)]SO4·5H2O;利用荧光光谱技术对该混配合物与牛血清白蛋白的(BSA)的相互作用进行了研究分析。结果表明:混配合物与BSA相互作用,可以使BSA产生内源性荧光猝灭,猝灭机理为静态猝灭,表观猝灭常数为K q=1.2×1012L·mol-1·s-1,结合常数为K=9.7×103L/mol,结合位点数为n=0.966。更多还原

【Abstract】 The binding interaction between the mixed complexes([Cu(Glu·Arg) ]SO4·5H2O) and bovine serum albumin(BSA) was investigated by fluorescence spectroscopy.The mixed complexes was designed and synthesized with Cu(Ⅱ),glutamate(Glu) and arginine(Arg).The mixed complexes have powerful ability to quench the BSA fluorescence via a static quenching mechanism and quenching rate constant is1.2 × 1012L·mol- 1·s- 1and binding constant is 9.7 × 103L/mol and the number of binding site is 0.966.更多还原