【摘要】 利用十六烷基三甲基溴化铵(CTAB)/正己醇/异辛烷反胶束萃取阿魏酸酯酶.考察pH值、离子强度、CTAB浓度对阿魏酸酯酶的CTAB/正己醇/异辛烷反胶束萃取及其反萃取的影响.结果表明:萃取的最佳条件为酶液透析24h,pH值为12,萃取液为25mmol.L-1 CTAB的正己醇/异辛烷溶液(体积比为1∶5),萃取率接近100%;反萃取的最佳条件为pH值为7的0.20mol.L-1 KCl溶液,反萃取率可达79%.纯化后比活为356.7μkat.g-1,纯化倍数为6.9,SDS-PAGE电泳显示两条带.更多还原

【Abstract】 The cetrimonium bromide/hexanol/isooctane reverse micelles extraction and backward extraction were used to purify feruloyl esterase.The effects of pH,ionic strength and cetrimonium bromide(CTAB) concentration on the cetrimonium bromide/hexanol/isooctane reverse micelles extraction and backward extraction were studied.The results showed that the extraction efficiency was nearly 100% when the optimal extraction conditions were used as followed: enzyme dialysis time 24 h,pH 12 and CTAB concentration in hexanol/isooctane solution(volume ratio 1∶5) 25 mmol·L-1.The optimal condition of backward extraction was 0.20 mol·L-1 KCl with pH 7,and the efficiency of backward extraction was 79%.After extraction and backward extraction of the enzyme,specific activity was 356.7 μkat·g-1,purification fold was 6.9,and SDS-PAGE electrophoresis showed two bands.更多还原