【摘要】 目的:研究半边旗提取物5F对乳癌细胞MDA-MB-231培养物(乳癌细胞培养物)诱导的人脐静脉内皮细胞(HUVEC)血管生成潜能的影响及其作用机制。方法:MTT法检测不同浓度5F对乳癌细胞培养物诱导的HUVEC增殖的抑制作用;Transwell小室法检测不同浓度5F对乳癌细胞培养物诱导的HUVEC穿膜能力和趋化性运动能力的影响;细胞-基质黏附实验检测5F对乳癌细胞培养物诱导的HUVEC黏附能力的影响;RT-PCR、Western blot法检测不同浓度5F对乳癌细胞培养物诱导的HUVEC KDR、Flt-1 mRNA和蛋白表达的影响。结果:不同浓度5F处理6、24 h后对乳癌细胞培养物诱导的HUVEC的增殖有一定程度的抑制作用(F组间=64.852,F时间=131.393,P<0.001)。20、40、80μmol/L 5F可抑制乳癌细胞培养物诱导的HUVEC体外穿膜能力、趋化性运动能力和黏附基质能力(F=48.206、147.613、22.081,P均<0.001)。不同浓度5F作用于乳癌细胞培养物诱导的HUVEC 24 h后,下调HUVEC KDR、Flt-1 mRNA和蛋白的表达(mRNA:F=86.381、79.175;蛋白:F=36.621、127.910,P均<0.001)。结论:5F抑制乳癌细胞培养物诱导的HUVEC体外增殖、穿膜能力、趋化性运动能力和黏附基质能力,其机制可能与下调细胞KDR mRNA和蛋白的表达水平有关。更多还原

【Abstract】 Aim:To investigate the effect and its possible mechanisms of Pteris semipinnata L extract( 5F) on angiogenic abilities of human umbilical vein endothelial cells( HUVEC) induced by the culture of human high metastatic breast cancer MDA-MB-231 cells. Methods:MTT assay was used to evaluate the cell proliferation of HUVEC induced by the culture of MDA-MB-231 cells after being treated by 5F for 6 h and 24 h. The effect of 5F on invasion and migration of HUVEC induced by the culture of MDA-MB-231 cells was measured by transwell chamber assay. The adhesive potential of HUVEC cells induced by the culture of MDA-MB-231 cells was tested by cell-matrix adhesion assay. RT-PCR and Western blot were applied to detect the expression levels of KDR,Flt-1 mRNA and protein in HUVEC induced by the culture of MDAMB-231 cells. Results:5F inhibited the proliferation of HUVEC induced by the culture of MDA-MB-231 cells after 6 h and 24 h treatment( F group= 64. 852,F time= 131. 393,P < 0. 001). 20,40 and 80 μmol / L 5F significantly inhibited the invasion,migration and adhesion to matrigel of HUVEC induced by the culture of MDA-MB-231 cells( F = 48. 206,147. 613,22. 081,P < 0. 001). After 24 h treatment,5F down-regulated the expressions of KDR,Flt-1 mRNA and protein( mRNA:F =86. 381,79. 175; protein:F =36. 621,127. 910,P <0. 001) induced by the culture of MDA-MB-231 cells.Conclusion:5F could inhibit the proliferation,invasion,and migration of HUVEC induced by the culture of MDA-MB-231cells and adhesion to matrix of HUVEC,which may be involved in its down-regulation of the expression of KDR.更多还原