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人乳头瘤病毒16型L1基因乳酸杆菌表达载体的构建
Construction of expression vector of human papillomavirus 16 L1 gene in lactobacillus
【摘要】 目的:构建含人乳头瘤病毒16型(HPV16)L1基因的乳酸杆菌分泌表达载体。方法:从培养的CasKi细胞中提取DNA为模板,经PCR得到HPV16L1衣壳蛋白基因,将其连接入乳酸杆菌分泌表达载体pVE5523质粒中,并转化入Top10菌中保存。结果:构建的HPV16L1-pVE5523重组表达载体经PCR鉴定正确;获得的目的基因片段经测序与GenBank公布的HPV16L1基因序列符合。结论:成功构建了HPV16L1-pVE5523表达载体。
【Abstract】 Aim:To construct the expression vector of human papillomavirus 16(HPV16) L1-pVE5523.Methods:The total DNA extracted from the CasKi cells was used as template for the amplification of HPV16 L1 gene by using PCR.The amplified products were ligated with expression plasmid pVE5523 of lactobacillus.Then the recombination product HPV16 L1-pVE5523 was preserved in Top10 bacteria.Results:The recombination product HPV16 L1-pVE5523 checked by PCR was correct.The DNA sequence of the recombination product was identical with HPV16 L1 gene published in GenBank.Conclusion:The expression vector HPV16 L1-pVE5523 has been constructed successfully.
【Key words】 human papillomavirus 16； L1 gene； lactobacillus； pVE5523； expression vector；
- 【文献出处】 郑州大学学报(医学版) ,Journal of Zhengzhou University(Medical Sciences) , 编辑部邮箱 ,2013年01期
- 【分类号】R346
- 【被引频次】7
- 【下载频次】217