【摘要】 目的:构建杜氏盐藻叶绿体转化载体pUC-chlN1622-氯霉素乙酰转移酶(CAT)。方法:以光非依赖性的原叶绿素酸酯还原酶chlN基因为同源片段,以CAT抗性基因为选择标记,构建盐藻叶绿体转化载体pUC-chlN1622-CAT,并通过电击法转入野生型盐藻细胞,筛选转化藻株。结果:在氯霉素浓度为300mg/L的选择压力下,野生型盐藻10d左右死亡,转化藻仍正常生长,得到表达氯霉素抗性的盐藻转化株。结论:以chlN基因作为同源片段构建盐藻叶绿体转化载体是可行的。更多还原

【Abstract】 Aim:To construct the transformation vector pUC-chlN 1622-chloramphenicol acetyltransferase(CAT) for the chloroplast of Dunaliella salina(D.salina).Methods:By using CAT gene as selective marker,and the chlN,one of light-independent protochlorophyllide reductase genes,being used as homologous segment,a transformation vector pUC-chlN 1622-CAT for the chloroplast of D.salina was constructed,which was transferred into the cells of normal D.salina,using the electroporation.Results:The transformants of D.salina survived under the selection pressure of chloramphenicol at 300 mg/L,while the wild type cells died at about 10 days after electroporation.The transformants had resistance to chloramphenicol.Conclusion:It is available to construct transformation vector for D.salina chloroplast using the chlN gene as homologous segment.更多还原