【摘要】 为探讨川白芷不同器官内源激素的提取纯化和分析测定,在前人研究的基础上,建立了一种从川白芷不同器官组织中快速提取纯化并同步测定4种植物内源激素(玉米素、赤霉素、3-吲哚乙酸和脱落酸)的高效液相色谱分析方法。样品经80%甲醇提取后低温浓缩,最后用甲醇定容。采用Tigerkin C18柱(150mm×4.6mm,5μm)以甲醇—0.6%乙酸(50:50)为流动相进行高效液相色谱分离,PDA光电二极管阵列检测器检测,采用峰面积外标法(ESTD)进行定量。结果表明,该方法具有简单快捷,准确灵敏等优点,适合对川白芷内源激素进行快速同步测定。更多还原

【Abstract】 A simple and rapid method using High Pressure Liquid Chromatography(HPLC) for the simultaneous determination of four representative endogenous hormones including Gibberellic acid(GA3),Zeatin(ZT),Indoleacetic acid(IAA) and Abscisic acid(ABA) in Angelica dahurica.was described.Endogenous hormones were extracted using ethanol solution(80%,V/V) under low-temperature condition.Then the extracting sample was separated by HPLC with a C18 ODS column(150 mm×4.6mm,5μm) using methanol-0.6% acetic acid(50:50,v/v) as mobile phase.The signal was detected by Photodiode Array(PDA),and the external standard method(ESTD) was employed for quantitative analysis.The experimental results demonstrated this method was an accurate and high-separation-efficiency technique to analyze trace amounts of the four endogenous hormones in various organs of Angelica dahurica.更多还原