【摘要】 测定小菜蛾Cry1Ac抗性品系(Cry1Ac–R)、Cry1Ac敏感品系(Cry1Ac–S)3种解毒酶的活性。结果表明,小菜蛾抗性品系的谷胱甘肽转移酶活性高于敏感品系;敏感品系的羧酸酯酶和乙酰胆碱酯酶的活性高于抗性品系,但2品系的解毒酶活性无显著差异。测定小菜蛾Cry1Ac抗性品系、敏感品系及Bt抗性品系(Bt–T2)消化酶活性,结果小菜蛾敏感品系海藻糖酶、蔗糖酶活性均最高,Bt抗性品系最低,但各品系的2种消化酶活性之间差异不显著;小菜蛾敏感品系蛋白酶活性最高,抗性品系最低,各品系的蛋白酶活性差异极显著。更多还原

【Abstract】 The activities of gultathione S transferases(GSTs), carboxylesterase(CarE) and Acetylcholinesterase(AChE)in laboratory strains of the Plutella xylostella have been examined.The result shows that the activity of GSTs of the resistant strain(Cry1Ac–R), Cry1Ac is higher than that of the susceptible strain(Cry1Ac–S). The activities of Care and AChE of the susceptible strain are higher than that of the resistant strain, but there is no obvious difference of detoxification enzymes activity between the two strains. The activities of digestive enzyme of the resistant strain,(Cry1Ac)the susceptible strain and the Bt resistant strain(Bt–T2) have also been examined. The activities of trehalase and sucrase of the susceptible strain are the highest, those of the Bt resistant strain are the lowest, but there is no obvious difference between the two strains in the two digestive enzymes Protesease activity of the susceptible strain of the Plutella xylostella is the highest, while the protesease activity of its resistant strain is the lowest, and the difference of protesease activity between these strains is very obvious.更多还原