【摘要】 为了制备伊维菌素完全抗原,试验对伊维菌素进行结构改造,使之成为带活性基团的半抗原,并进行质谱鉴定,然后分别采用N-羟基琥珀酰亚胺酯(NHS)法将半抗原与牛血清白蛋白(BSA)耦联,耦联物作为免疫抗原;采用混和酸酐法将半抗原与卵清白蛋白(OVA)耦联,合成包被抗原。结果表明:耦联成功,耦联物的结合比分别为31.6和22.6。说明试验成功制备了伊维菌素完全抗原。更多还原

【Abstract】 To prepare the complete antigens of ivermectin. The structure of ivermectin was changed to become the haptens with activity group, which were identified by mass spectrum. The haptens were conjugated to a bovine serum albumin( BSA) carrier protein as immunogen using N- hydroxysuccinimide ester method( NHS),and conjugated to the ovalbumin OVA to synthesize the coating antigen using mixed acid anhydride method. The results showed that the conjugation was successfully achieved,and the ratios of conjugation were 31. 6 and 22. 6,respectively. It indicates that the complete antigens of ivermetin are successfully prepared.更多还原