【摘要】 目的:评价三种梅毒螺旋体IgM抗体检测方法在献血员筛查中的意义。方法:收集经过TPPA实验确诊为梅毒感染的献血员血浆标本150例,用胶体金、ELISA、WESTEN-BLOT方法进行TP-IgM检测。结果:一期梅毒、二期梅毒、潜伏梅毒的TP-IgM抗体阳性率,ELISA检测高于胶体金5%,胶体金、ELISA检测均高于WEST-EN-BLOT 5～10%。三种检测TP-IgM方法在不同时期梅毒组中,检测阳性率差异有统计学意义(P<0.05)。结论:不同检测方法对梅毒螺旋体IgM抗体检测的阳性率存在差异,快速检测胶体金法适用于快速初筛,ELISA检测适合于最终确诊。更多还原

【Abstract】 Objective:To evaluate the significance of three Treponema pallidum IgM antibody detective assays among blood donors.Methods:One hundred and fifty blood donors who were diagnosed as syphilis Infectors by TPPA were recruited and tested by Colloidal Gold,ELISA,and Western-blot for detection of TP-IgM.Results:The positive rate of ELISA in detection of TP-IgM was 5% higher than that of Colloidal Gold,and was 10% higher than that of Western-blot among different stages of Syphilis infection.The differences were significant(P<0.05).Conclusions:There were differences among the positive rates of different assays.Rapid Colloidal Gold assay can be used as a screening test,whereas ELISA can be used as a standardize test.更多还原