【摘要】 目的:探讨利用RNA干扰(RNA interference,RNAi)技术来抑制核心结合因子(core binding factor α1,Cbfa1)表达,从而阻断软骨细胞肥大分化目的。方法:构建沉默SD大鼠核心结合因子基因的腺病毒(Ad-Cbfa1-siRNA)。利用维甲酸及IL-1α促进软骨细胞肥大分化,研究Ad-Cbfa1-siRNA对Cbfa1表达的抑制作用。利用Ⅱ型胶原、Ⅹ型胶原免疫组化的方法以及RT-PCR比较各组之间Cbfa1的表达情况来分析软骨细胞的肥大分化情况。结果:结果显示,软骨细胞经维甲酸和IL-1α诱导后,Cbfa1的表达量明显增加,经统计学分析,P<0.05,具有统计学意义。阴性对照病毒组Cbfa1的表达量明显高于Ad-Cbfa1-siRNA组,经统计学分析,P<0.05,具有统计学意义。结论:利用RNAi技术能够明显的抑制Cbfa1的表达,从而阻断软骨细胞的肥大分化。更多还原

【Abstract】 Objective:We used RNAi technology to suppress Cbfa1 expression, to achieve the purpose of blocking the hypertrophic diferentiation ofchondrocytes Method:We constructed adenovirus containingsiRNAagainst Cbfa1(Ad-Cbfa1-siRNA). We used retinoic acid and IL-1αto induce hypertrophic differetiation of chondrocytes,and then We used Ad-Cbfa1-siRNA to inhibits the hypertrophic differentiation ofchondrocytes .We used immunohistochemistry to detect the type Ⅱand typeⅩcollagen and RT-PCR to analyze the expression of Cbfa1. Results:The result of RT-PCR showed thatthe expression of Cbfa1 was significantly increased after using the retinoic acid and IL-1α.The expression of Cbfa1 in control virus group was significantly higher than Ad-Cbfa1-siRNA group. Conclusion:In vitro,it is likelythat the inhibition ofCbfa1 byRNAi could be a powerful waytoprevent the hypertrophic differentiation ofchondrocytes .更多还原