【摘要】 研究拟香味菌Y6(Myroides odoratimimus strain)高盐条件下降解硝基苯的特性,拟解决高盐工业废水中硝基苯难降解问题.利用高效液相色谱测定硝基苯含量,分析高盐条件下温度、pH、硝基苯初始浓度、接种量、共代谢物等因素对菌Y6降解硝基苯特性的影响.结果表明,降解最佳条件为:pH值6,温度28℃,接种量D600=1,NaCl质量分数7%.在最佳降解条件下,菌株168 h内对100 mg·L-1和200 mg·L-1硝基苯降解率分别达到97.5%和65.7%.添加葡萄糖、淀粉和丙三醇对硝基苯降解均有促进作用,加入800 mg·L-1葡萄糖,其降解率达到93.3%(168 h,200 mg·L-1硝基苯).菌株Y6可用于硝基苯高盐工业废水的生物修复,这是首次报道耐盐拟香味菌降解硝基苯.更多还原

【Abstract】 Aimed at efficient remediation of nitrobenzene-contaminated saline wastewater,the nitrobenzene-degrading characteristics of a Myroides odoratimimus strain Y6 were studied and analyzed.The effects of temperature,pH,initial concentration of nitrobenzene,inoculum concentration and culture type on the biodegradation of nitrobenzene by strain Y6 under saline conditions were studied.Strain Y6 showed the highest efficiency of nitrobenzene degradation in 7% NaCl(mass fraction).The optimal conditions for the biodegradation of nitrobenzene by strain Y6 were at pH 6.0,28℃ and D600=1.With initial concentrations of 100 and 200 mg·L-1,97.5% and 65.7% of nitrobenzene were transformed after 168 h in 7% NaCl,respectively.Three kinds of additional compounds,glucose,starch and glycerin were tested to choose a favorite carbon source for the co-metabolism of strain Y6.The results showed that all these three compounds could promote nitrobenzene biodegradation and cell growth.And the rate of degradation could increase to 93.3%,with 800 mg·L-1 glucose as the primary substrate.These results suggest that strain Y6 could be a potential candidate for treating nitrobenzene-contaminated saline wastewater.To date,this is the only study on the degradation of nitrobenzene by M.odoratimimus.更多还原