【摘要】 研究了克雷伯氏菌在无机盐培养基和去离子水中对三苯基锡(TPhT)的降解性能,考察了不同时间TPhT降解率与各离子释放量的变化关系,为论证有机锡的微生物降解机理提供实验依据.5.0 g.L-1菌体处理120 h后,3.0 mg.L-1TPhT在无机盐培养基和去离子水体系中的降解率分别高达77.3%和60.9%.在TPhT降解过程中,TPhT会促进菌体离子的释放,加剧菌体的内陷并增加凋亡细胞的数量.TPhT的降解率与NH4+、Na+、PO34-、SO24-、NO2-的释放在0.01水平下显著正相关,Na+、NH4+、Ca2+的释放规律符合准二级动力学模式.XPS分析证明菌体降解TPhT 120 h前后细胞表面元素和基团没有发生改变,但TPhT则发生了转化.更多还原

【Abstract】 The correlation between TPhT biodegradation and ion release by Klebsiella pneumoniae was investigated.After degrading by 5 g · L-1 of Klebsiella pneumoniae for 120 h,the removal rate of TPhT in mineral salts medium and deionized water were up to 77.3% and 60.9%,respectively.The exposure to TPhT enhanced ions release and changed the cellular morphology inducing the apoptosis of some cells.The correlations between TPhT degradation efficiencies and the concentrations of NH+4,Na+,PO3-4,SO2-4,NO-2,Cl-and NO-3 were significant at the 0.01 level.After degradation by K.pneumonia for 120 h,X-ray photoelectron spectroscopy observation revealed that TPhT had no obvious impact on the elements and groups of the cellular surface.However,TPhT was degraded into inorganic tin by K.pneumonia.更多还原