【摘要】 本实验通过对8-细胞期2n或4n胚胎进行单细胞显微注射单个IPS,结果可以获得高嵌合率的胚胎,2n和4n胚胎的嵌合率分别达到86.0%±8.4%和70.4%±18.1%,相比囊胚注射更易操作,此实验为进一步制备高嵌合率的小鼠做准备.更多还原

【Abstract】 This experiment seeks to microinject a single IPS into 8-cell stage 2n or 4n embryo for obtaining high chimeric mouse blastocyst.The results show that,compared to blastocyst microinjection,single cell injection in the 8-cell stage is easier to execute and yields better chimeric rates.The author randomly chose 10 embryos from each of chimeric embryos(2n/2n and 2n/4n) to calculate blastosphere chimeric rate,which was above 70%(2n/2n embryos: 86.0%±8.4%,P=0.05;2n/4n embryos: 70.4%±18.1%,P=0.05) on average.This study thus provides valuable information for creating highly chimeric mice.更多还原