【摘要】 开发具有契合肿瘤细胞尺寸的微台阶结构的微流控芯片以将肿瘤细胞从正常人血细胞中分选并捕获出来.结合微加工技术,先后两次对同一块玻璃基底进行刻蚀,改变两次刻蚀的图样与时间最终与盖板间形成高度为10μm的微台阶结构,通过玻璃基底与有机高聚物PDMS(Polydimethylsiloxane)盖板的键合制作出微流控芯片.利用具有微台阶结构的芯片,悬浮于磷酸盐缓冲盐溶液中的肿瘤细胞(MCF-7)被全部捕获在微台阶结构内,尺寸小于台阶的正常人血细胞(红细胞)流过台阶未被捕获,实现了将肿瘤细胞从正常血细胞中分选并捕获.捕获在芯片中的肿瘤细胞都具有活性.芯片整体透明,肿瘤细胞捕获过程不需要进行化学修饰等预处理.更多还原

【Abstract】 A novel microfluidic chip with a size-special micro footstep structure inside was fabricated for sorting and capturing tumor cells from normal human blood cells.Combined with micromachining technology,10μm-height micro footstep structure was formed on the glass substrate after treated with two serial different etching procedures.And the chip was gained when the glass substrate and polydimethylsiloxane(PDMS)ceiling sealed together.Utilizing the special footstep structure within this chip,tumor cells(MCF-7)suspended in buffer could be totally captured inside the footstep,while all normal human blood cells(red blood cells)flew through into the waste part.Thus tumor cells were sorted and captured from red blood cells successfully.Whole captured tumor cells maintained their viability.This transparent microfluidic chip facilitates real-time monitoring,and no extra physical manipulations or chemical pre-treatment was needed.更多还原