【摘要】 目的通过检测转录因子KLF5蛋白和细胞周期素D1(Cyclin D1)、增殖细胞核抗原(PCNA)、P53蛋白在结肠癌组织中的表达,对KLF5在结肠癌细胞增殖过程中的意义进行探讨。方法对48例结肠癌和30例癌旁组织石蜡标本进行免疫组化染色,检测KLF5与Cyclin D1、PCNA、P53在两种组织中的表达情况,并分析其关系。结果结肠癌组织中KLF5、Cyclin D1、PCNA表达均强于癌旁组织(均P<0.05),P53在两种组织中表达无明显差异(P>0.05)。相关分析显示,结肠癌组织中KLF5与Cyclin D1之间、KLF5与P53之间表达存在正相关关系(P均<0.05);Cyclin D1与PCNA表达也存在正相关关系(P<0.05),Cyclin D1与P53、PCNA与P53之间表达均无明显相关关系(P>0.05)。结论结肠癌组织中KLF5表达上调,这与KLF5可能通过调节CyclinD1、P53表达而参与结肠癌细胞增殖有关。更多还原

【Abstract】 Objective To investigate the significance of the expressions of Krüppel-like factors 5 (KLF5),Cyclin D1,proliferating cell nuclear antigen (PCNA),P53 during cell proliferation in colon cancer.Methods The expression levels of KLF5,Cyclin D1,PCNA,P53 were detected by immunohistochemistry in 48 cases of colon cancer paraffin-embedded specimens and 30 cases of carcinoma-adjacent tissues,and the correlation among them was analyzed.Results The expression levels of KLF5,Cyclin D1,PCNA in colon cancer tissues were significantly higher than those in carcinoma-adjacent tissues (P<0.05),howecer,there were no significant differences in the expression levels of P53 between the two kinds of tissues (P>0.05).The results of correlation analysis showed that KLF5 was positively correlated to Cyclin D1,so to P53 (P<0.05);Cyclin D1expression was positively related to that of PCNA (P<0.05),however,there was no correlation between Cyclin D1 and P53 and between PCNA and P53(P>0.05).Conclusion The expression levels of KLF5 are up-regulated in colon cancer tissues,which may be involved in the cell proliferation of colon cancer by regulating the expression levels of Cyclin D1 and P53.更多还原