【摘要】 目的建立柱前衍生-高效液相色谱法测定血清样品中丙戊酸钠的含量。方法以Diamonsil C₁8（4.6×250mm 5μm）为色谱柱,流动相为甲醇-水（87:13,V/V）,检测波长为245nm;流速为1.0mL/min;柱温25℃样品酸化提取后经α-溴代苯乙酮衍生化后分析,环己烷羧酸为内标。结果标准曲线线性相关系数（r）为0.999 3,线性范围15.0¹80.0μmL,平均回收率为103.2%,日内、日间相对标准差均小于10%,最低检出浓度为0.3μmL。结论该方法简便、准确,适合于临床血药浓度监测。更多还原

【Abstract】 Objective To establish a method for determining sodium valproate in serum by high performance liquid chromatography(HPLC) after pre-column derivatization.Methods The analytical column was Diamonsil C18 column(250mm ×4.6mm,5μm).The mobile phase consisted of methanolwater(87:13,V/V) at the flow rate of 1.OmL/min.The detection wave length was set at 245nm and the column temperature was set at 25℃.The samples were extracted after being acidified,then derived withα-bromoacetophenone before analysis.Cyclohexane carboxylic acid was used as the internal standard.Results The liner correlation of the standard curvilinear equation was r =0.999 3 with a linear range of15.0-180.00μg/mL.The mean relative recovery was 103.2%.The intra-day and the inter-day relative standard deviation were less than 10%.The lowest limit of detection of valproate was 0.3 μg/mL.Conclusion This method was convenient and accurate for the blood concentration monitoring of therapeutic drugs.更多还原