【摘要】 目的:探讨博来霉素诱导小鼠肺间质纤维化过程中乳腺退化蛋白39(BRP-39)的表达水平变化。方法:取7-8周龄C57BL/6小鼠20只,随机分为博来霉素模型组和正常对照组,每组各10只;博来霉素组采用气管滴入5g/L博来霉素溶液25μl,而对照组则予以等量生理盐水。处理后14d,分别取两组小鼠肺组织应用HE和Masson染色,观察肺组织改变情况;ELISA法检测羟脯氨酸含量;免疫组织化学法和Western blot法分别检测BRP-9蛋白表达水平。结果:博来霉素处理后,镜下HE染色可见肺泡间隔轻度增宽、病灶区域巨噬细胞等炎性细胞浸润的改变,且肺组织损伤分布较广,纤维沉积明显;且与正常对照组比较,Masson蓝染胶原也明显增多;此外,博来霉素组小鼠肺组织中羟脯氨酸含量明显高于正常对照组(P<0.05);免疫组织化学检测结果表明,博来霉素组BRP-39广泛分布于炎症细胞和纤维化肺组织中且阳性表达水平显著高于正常对照组(P<0.05);Western blot结果亦显示,肺间质纤维化可引起BRP-39表达含量显著增加,蛋白表达量可达正常对照组的2.35倍(P<0.05)。结论:BRP-39在肺纤维化模型中升高,提示其可能在肺间质纤维化过程中发挥重要作用。更多还原

【Abstract】 Objective:To explore the expression of BRP-39 in bleomycin-induced interstitial pulmonary fibrosis in mice.Methods:Twenty C57BL/6mice,aged 7-8 weeks,were randomly divided into bleomycin model group and normal control group(n=10).Interstitial pulmonary fibrosis model was prepared by tracheal instillation with 25μl bleomycin(5g/L),while the mice in the control group received equivalent normal saline.After 14 days,the lung tissue specimens were stained with HE and Masson respectively to observe the pathological change.The level of hydroxyproline was determined by ELISA assay.The expression of BRP-39 was detected with immunohistochemistry and Western blot.Results:After bleomycin treatment,in the HE staining assay,alveolar septum was slightly widened,and the lesions in the region of macrophages and other inflammatory cell infiltration were changed with wider distribution of lung tissue damage,and the fibrin deposition was severe.Compared with normal control group,Masson blue dye collagen was significantly increased.Hydroxyproline content in bleomycin-treated mice lung tissue was significantly higher than that of control group(P<0.05).Immunohistochemistry results showed that BRP-39 in bleomycin group was widely distributed in inflammatory cells and fibrosis lung tissue,and the positive expression was significantly higher than that in the control group(P<0.05).Western Blot results also showed that pulmonary fibrosis could cause significantly increased BRP-39 expression,and protein expression was 2.35 times than in normal control group(P<0.05).Conclusion:BRP-39 is elevated during the progress of interstitial pulmonary fibrosis.更多还原