【摘要】 为了观察不同剂量食欲素A在不同时间对绵羊黄体化颗粒细胞OX1R表达的影响,在绵羊黄体化颗粒细胞中添加不同剂量(0.05,0.2,0.5,1μg/mL)食欲素A,分别于刺激后0,2,6,12 h提取细胞总RNA,运用RT-qPCR方法检测了OX1R mRNA相对表达量变化。结果表明,OX1R mRNA分布于绵羊黄体化颗粒细胞中;添加高剂量(1μg/mL)食欲素A在2 h显著性促进OX1R mRNA表达(P<0.01),随后这种作用会逐渐减弱;添加低剂量(0.05,0.2,0.5μg/mL)食欲素A后12 h时会显著促进OX1R mRNA表达(P<0.01),且0.2μg/mL剂量组促进作用更为显著。食欲素A诱导并上调OX1R mRNA的表达,高剂量(1μg/mL)食欲素A可以迅速诱导其表达,而低剂量(0.05,0.2,0.5μg/mL)食欲素A诱导作用较为缓慢,且高剂量(1μg/mL)组食欲素A同低剂量(0.05,0.2,0.5μg/mL)组相比,上调作用更加迅速。更多还原

【Abstract】 To observe the effect of orexin A on the expression of OX1R in ovine luteinized granulosa cells in vitro,the cells were treated with different concentrations( 0. 05,0. 2,0. 5,1 μg / mL) of orexins A. Then the total RNA was extracted from the cells after treated in 0,2,6,12 h,and the mRNA expression levels of OX1R were evaluated using reverse transcription quantitative PCR. In result,orexin A induced release of OX1R in ovine luteinized granulosa cells. In high concentration( 1 μg / mL) group,the OX1R mRNA expression level was increased to the highest in 2 h( P < 0. 01) and gradually weakened since then; in low concentration( 0. 05,0. 2,0. 5 μg / mL) group, the OX1R mRNA expression level was increased in 12 h and the expression level of 0. 2 μg / mL group was increased to the highest in 12 h( P < 0. 01). Orexin A induced expressions of OX1R mRNA,the group of 1 μg / mL was more rapid up regulation compared with the low concentration( 0. 05,0. 2,0. 5 μg / mL) group.更多还原