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结球甘蓝抽薹开花时间性状的QTL定位及分析

QTL Mapping and Analysis on Bolting and Flowering Time in Cabbage

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【作者】 朱洪运田多成颉建明简元才康俊根

【Author】 ZHU Hong-yun;TIAN Duo-cheng;XIE Jian-ming;JIAN Yuan-cai;KANG Jun-gen;Beijing Vegetable Research Center,Beijing Academy of Agriculture and Forestry Sciences,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China),Ministry of Agriculture;Gansu Agricultural University;

【机构】 北京市农林科学院蔬菜研究中心,农业部华北地区园艺作物生物学与种质创制重点实验室甘肃农业大学农学院

【摘要】 在已构建的结球甘蓝AFLP、SSR和SRAP标记高密度遗传图谱的基础上,运用MapQTL 4.0软件对结球甘蓝F2群体抽薹、开花时间两性状分别进行QTL定位和分析。最终检测到2个控制甘蓝抽薹时间性状的QTL(qbt-3-2、qbt-9-1),以及一个同开花时间性状相关的QTL(qft-9-1),分别位于连锁群LG3与LG9上,这3个QTL均为增效位点,共解释甘蓝抽薹、开花时间性状变异的17.5%,22.7%;同时得到与QTLs共分离的2个分子标记E46M52-5、me26-em13-1,均可作为辅助选育甘蓝耐抽薹品种的标记使用,这将为结球甘蓝耐抽薹品种的选育提供理论依据。

【Abstract】 Based on the Cabbage genetic linkage map which had been constructed previously,QTL mapping and analysis related to bolting and flowering time in the individuals of F 2 were conducted using MapQTL 4.0.Two QTL(qbt-3-2,qbt-9-1) located on LG3 and LG9 controlling the traits of bolting time and one QTL(qft-9-1) located on LG9 controlling the traits of flowering time in cabbage were detected.The three QTLs are all the sites of additive effect,17.5%,22.7% of variation that can be explained respectively.Meanwhile,two molecular markers(E46M525,me26-em13-1) corresponding with the QTLs could be used for M-AS of breeding for bolting tolerance.It will provide the basis of theoretical for the resistant bolting varieties breeding.

【基金】 “十二五”国家科技支撑计划项目(2012BAD02B01;2012BAD50G01);国家“863”项目(2012AA1001);北京市科委项目(D121100003412001)
  • 【文献出处】 华北农学报 ,Acta Agriculturae Boreali-Sinica , 编辑部邮箱 ,2013年05期
  • 【分类号】S635.1
  • 【被引频次】10
  • 【下载频次】194
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