【摘要】 以水为溶剂采用微波辅助辐射法提取了马尾松树皮总黄酮。以芦丁标准品为对照品,用分光光度法测定马尾松树皮总黄酮的含量;探讨了料液比、微波辐射功率、微波辐射时间、浸提时间、浸提温度等因素对马尾松树皮总黄酮提取率的影响,通过正交实验得出微波辅助提取马尾松树皮总黄酮的最佳条件为:料液比1∶25(g∶mL)、微波辐射功率350W、微波辐射时间3×40s(间歇3次)、浸提时间4h、浸提温度60℃,在此条件下,总黄酮提取率达到3.08%。微波辅助下以水为溶剂提取马尾松树皮总黄酮高效、快速、经济,具有较好的实用价值。更多还原

【Abstract】 Total favonoids in Pinus massoniana bark was extracted by microwave-assisted extraction using water as solvent.With rutin standard as reference substance,flavonoids content in Pinus massoniana bark was determined by spectrophotometry.The effects of solid-liquid ratio,microwave irradiation power,microwave irradiation time,soaking time,soaking temperature on extraction rate of total flavonoids in Pinus massoniana bark were studied.The optimum conditions of microwave-assisted extraction of total flavonoids in Pinus massoniana bark were obtained through single factor experiment and orthogonal experiment as follows:solid-liquid ratio of 1∶25(g∶mL),microwave irradiation power of 350 W,microwave irradiation time of 3×40 s(intermittent 3 times),soaking time of 4 h,soaking temperature of 60 ℃.Under above conditions,the extraction rate could reach 3.08%.Microwave-assisted extraction of total flavonoids in Pinus massoniana bark with water as solvent was rapid,efficient and economic.更多还原