【摘要】 目的了解肝功能正常、HBeAg阴性HBV感染的术前筛查患者乙肝大蛋白(HBV-LP)阳性情况,评判血清HBV-LP检测在这类乙型肝炎患者中的意义。方法使用ELISA法检测180例HBeAg阴性HBV感染的术前筛查患者HBV-LP、乙肝前S1(HBVpreS1),同时采用实时定量PCR法做HBVDNA水平检测。结果 180份HBeAg阴性HBV感染血清PreS1-Ag、HBV-LP和HBVDNA检测阳性率分别为21.1%、34.4%和32.7%,χ2检验显示三组检测阳性率差异有统计学意义(χ2=9.1457,P=0.0103),用Scheffe法做率的两两比较显示:PreS1-Ag检测阳性率明显低于HBV-LP和HBVDNA(P<0.05),而HBV-LP和HBVDNA检测阳性率差异无统计学意义(P>0.05)。HBV-LP和HBVDNA配对检测显示结果差异无统计学意义(χ2=0.1739,P=0.6767),同时相关性分析示良好相关性(χ2=95.655,P=0.0000,r=0.7217)。49例HBVDNAcopies/ml常用对数值与HBV-LP定量OD值的散点图显示正相关(r=0.6192)。结论对术前筛查HBeAg阴性乙型肝炎携带者,HBV-LP在反应病毒复制方面是一个优于HBVpreS1检测的良好指标,与HBVDNA检测有良好的相关性,在不能开展HBVDNA检测的基层单位可作为评价乙型肝炎传染性的一种替代与补充。更多还原

【Abstract】 Objective To detect serum hepatitis B virus large surface protein (HBV-LP) in preoperative screening in subjects with normal liver function and patients with HBeAg-negative hepatitis B infection, and to evaluate the significance of serum HBV-LP in the patients with hepatitis B. Methods HBV-LP, hepatitis B S1 (HBVpreS1) were measured by ELISA, and HBV DNA levels were detected by fluorescence quantitative PCR in 180 HBeAg-negative HBV patients in preoperative screening. Results In the 180 patients, the positive rates of PreS1-Ag, HBV-LP and HBV DNA were 21.1%, 34.4% and 32.7%, respectively. χ2 test showed that the positive rates of three groups had significant difference (χ2 =9.145 7, P=0.010 3). Scheffe test showed that the positive rate of PreS1-Ag detection was significantly lower than that of HBV-LP and HBV DNA (P<0.05), while no significant difference was found in the positive rates of HBV-LP and HBV DNA detection (P>0.05). HBV-LP and HBV DNA showed no significant differences in detection results (χ2 =0.173 9, P=0.676 7). At the same time, correlation analysis showed a good correlation between HBV-LP and HBV DNA detection (χ2 =95.655, P=0.0000, r=0.721 7). The scatter plot of 49 cases of HBV DNA copy number per ml (copies/ml) commonly used numerical and quantitative HBV-LP OD showed a positive correlation (r=0.6192). Conclusion HBV-LP, superior to HBVpreS1, is a good indicator in response to viral replication. There was a good correlation between HBV-LP and HBV-DNA detection, which can be used as a substitute and supplement of hepatitis B infection in hospital.更多还原