【摘要】 目的通过体外培养人牙髓干细胞(Human dental pulp stem cells,hDPSCs),观察重组腺病毒介导的表皮生长因子(Recombinant adenovirus mediated epidermal growth factor,rAd-EGF)转染至hDPSCs后对其凋亡的影响。方法 rAd-EGF转染hDPSCs后,采用RT-PCR检测EGF mRNA的表达情况,用流式细胞仪Annexin V-FITC/PI法检测hDPSCs的凋亡情况。结果在一定时间内,rAd-EGF转染入hDPSCs,实验组hDPSCs中EGF mRNA的表达水平相对于阴性对照组与空白组均明显上调(P<0.05),并且同阴性对照组与空白组比较,实验组hDPSCs体外凋亡指数明显降低(P<0.05)。结论 rAd-EGF转染入hDPSCs,EGF mRNA的表达量增加,在短期内对其凋亡具有抑制作用。更多还原

【Abstract】 Objective To observe the effect of recombinant adenovirus mediated epidermal growth factor (rAd-EGF) transfecting the human dental pulp stem cells (hDPSCs) on its apoptosis by culturing human dental pulp stem cells in vitro. Methods After used rAd-EGF to transfect hDPSCs, reverse transcription polymerase reaction (RT-PCR) was used to analyze the mRNA expression of EGF in hDPSCs, and flow cytometry Annexin V-FITC/PI was used to detect the effect of the apoptosis of hDPSCs. Results Within a certain time, rAd-EGF was transfected into hDPSCs. The EGF mRNA expression of hDPSCs in the experimental group were significantly up-regulated compared with the negative control group and blank control group (P<0.05). And compared with the negative control group and the blank group, the apoptotic index of hDPSCs in the experimental group was significantly lower (P<0.05). Conclusion The rAd-EGF could be transfected into hDPSCs successfully, and the EGF mRNA expression of hDPSCs was increased. In the short term, the rAd-EGF transfected into hDPSCs could inhibit apoptosis.更多还原