【摘要】 目的评估5-氨基酮戊酸光动力疗法(5-ALA-PDT)在体外对白念珠菌的抑制效应。方法以96孔细胞培养板为实验载体,加入白念珠菌悬液,分为治疗组和4个对照组,以氦氖激光为激发光源,ALA为光敏剂,使用DMSO作为ALA的溶剂兼穿透增强剂,进行ALA-PDT。结果治疗组白念珠菌的生长受到抑制,其MIC值为500mg/ml(50%),ALA浓度在200mg/ml(20%)时,开始使治疗组白念珠菌的生长受到抑制,且抑制作用对ALA的浓度呈剂量依赖关系。而对照组中白念珠菌的生长未受到抑制,四组的白念珠菌的CFU数量变化无明显差异。结论按本实验所使用的方法进行的ALA-PDT能有效地抑制白念珠菌。更多还原

【Abstract】 Objective To evaluate the inhibitory effect of 5-aminolevulinic acid Photodynamic therapy on Candida albicans in vitro. Methods 96-well cell culture plates were used for experimental carriers, and Candida albicans suspension was added to these wells, which were divided into experimental group and four control groups. With He-Ne laser as the excitation source, ALA as photosensitizer, DMSO as penetration enhancer and a solvent, ALA-PDT was performed. Results The growth of Candida albicans was inhibited in experimental group, with MIC of 500 mg/ml (50%). At concentration of 200 mg/ml (20%), ALA began to inhibit the growth of Candida albicans in experimental group, and ALA inhibited the growth of Candida albicans in a concentration-dependent way. The growth of Candida albicans was not inhibited in four control groups, and the CFU of Candida albicans showed no statistically significant difference between the groups. Conclusion ALA-PDT can effectively inhibit the growth of Candida albicans.更多还原