【摘要】 目的探讨多重PCR技术在检测无症状性尿道炎或宫颈炎患者中沙眼衣原体(Ct)、淋病奈瑟菌(Ng)、生殖支原体(Mg)、解脲脲原体(Uu)、人型支原体(Mh)以及阴道毛滴虫(Tv)的感染情况。方法选取2010年7月至2011年12月前来我院检查的216例无症状人群为研究对象,通过获取首段尿或宫颈拭子标本后进行多重PCR扩增。结果 216例研究对象中95例(43.98%)至少1种病原体阳性,7例(3.24%)同时检测出2种病原体,2例(0.92%)同时检测出3种病原体,1例(0.46%)同时检测出4种病原体。其中Ct、Ng、Mg、Uu、Mh,Tv在无症状人群中的检出率分别为8.33%(18/216)、0.92%(2/216)、0.92%(2/216),17.59%(38/216)、14.35%(31/216)和1.85%(4/216)。结论多重PCR仅需单份样本就能从无症状性尿道炎或宫颈炎患者中快速检测出6种病原体,由于此类人群容易被忽视,因此多重PCR技术在筛查性传播病原体中具有重要的意义。更多还原

【Abstract】 Objective To determine the prevalence of Chlamydia trachomatis (Ct), Neisseria gonorrhoeae (Ng), Mycoplasma genitalium (Mg), Ureaplasma urealyticum (Uu), Mycoplasma hominis (Mh), and Trichomonas vaginalis (Tv) in patients with asymptomatic urethritis or cervicitis by multiplex PCR. Methods Two hundred and sixteen people who came to our hospital for checkup from July 2010 to December 2011 were selected as subjects of the study. Multiplex PCR assays were performed with first voided urine samples or endocervical swabs. Results Among the 216 people, 95 (43.98%) showed positive result for at least one pathogen, 7 (3.24%) were positive for two different pathogens, 2 (0.92%) for three different pathogens and 1 (0.46%) for four different pathogens. The prevalence rates of Ct, Ng, Mg, Uu, Mh, Tv were 8.33% (18/216), 0.92% (2/216), 0.92% (2/216), 17.59% (38/216), 14.35% (31/216) and 1.85% (4/216), respectively. Conclusion Multiplex PCR could identify the prevalence rates of six pathogens and the overall proportion of urethritis or cervicitis in asymptomatic people with a single sample, which is of great significance to the screening of sexually transmitted diseases.更多还原