【摘要】 目的:探讨巨噬细胞移动抑制因子(macrophage migration inhibitory factor,MIF)的表达和巨噬细胞的浸润在口腔鳞癌的侵袭和转移中的作用及相互关系。方法:采用免疫组化SABC法分别检测MIF和CD68(标记巨噬细胞)在42例口腔鳞癌和10例口腔正常组织的表达情况,观察MIF表达与巨噬细胞计数之间的关系,并观察MIF阳性表达率及巨噬细胞计数在口腔鳞癌有无淋巴结转移患者及不同TNM分期患者的中的差异。结果:MIF的表达水平和巨噬细胞计数在口腔鳞癌中均显著高于正常组(P<0.01),肿瘤组织中MIF的表达与巨噬细胞计数之间呈正相关关系(r=0.542,P<0.01),MIF阳性表达率及巨噬细胞计数在口腔鳞癌有无淋巴结转移患者及不同TNM分期患者中差异具有统计学意义(P<0.05)。结论:MIF和巨噬细胞可能在口腔鳞癌的侵袭及转移中发挥重要作用,其机制可能与巨噬细胞作用于肿瘤细胞并协助上调肿瘤细胞中MIF的表达有关。更多还原

【Abstract】 Objective: To investigate the significance and relationship between the expression of macrophage migration inhibitory factor(MIF) and infiltration of macrophages in the process of invasion and metastasis in oral squamous cell carcinoma(OSCC).Methods: The immunohistochemical SABC method was used to detect MIF expression and CD68(for labeling macrophages) in 42 cases of OSCC and in 10 normal tissues,and so as to observe the relationship between MIF expression and macrophages infiltration,and to observe the variation of MIF expression and macrophages infiltration in OSCC patients with or without lymphnode metastasis,and in patients at different TNM stages.Results: MIF expression and macrophage counts in OSCC cases were significantly higher than in normal tissues(P < 0.01).There was a positive correlation between MIF expression and macrophage infiltration(γ = 0.542,P < 0.01).And the expression of MIF and macrophage counts were related to lymphnode metastasis and TNM stages(P < 0.05).Conclusion: Both MIF and macrophages may play an important role in the process of invasion and metastasis of OSCC,and the cellular activity may relate to macrophages which affect the tumor cells and up-regulate MIF expression更多还原