【摘要】 目的探讨不同染尘剂量大鼠矽肺模型血清中硅元素水平之间的剂量—反应关系并与可反映肺上皮细胞损伤和修复情况的细胞因子表面活性蛋白D(surface-active protein D,SP-D)进行比较。方法选取雄性Wistar大鼠按不同染尘剂量(10、30、50、70mg/ml)及不同时间点(染尘后1、3、5、7、14、21和28天)分为28个染尘组,取大鼠血液及肺组织,采用电感耦合等离子体质谱仪(ICP-MS)检测矽肺模型大鼠血清中硅元素含量,酶联免疫吸附测定法(ELISA法)定量测定大鼠血清中SP-D含量。结果随着染尘剂量的增加和时间的延长,模型组大鼠血清中硅元素的水平呈现逐渐增高的趋势;SP-D含量于造模后即开始增加,与对照组比较随染尘剂量及时间的增加呈逐渐增高然后下降的趋势。结论矽肺模型大鼠血清中硅元素水平在早期低剂量染尘时即明显升高,且早于SP-D的变化,二者水平均随染尘剂量和时间的不同而变化。提示,血清中硅元素和SP-D水平变化可能为矽肺的早期诊断提供生物学指标,具有一定的意义及价值。更多还原

【Abstract】 Objective To explore the dose-response relationship of the serum silicon level and SP-D in silica exposed rats.Methods Rats were randomly divided into 28 groups according to different SiO2 doses(10,30,50 and 70 mg/ml) and different exposure periods(1,3,5,7,14,21 and 28 days).The serum silicon levels were determined by inductively coupled plasma mass spectrometry(ICP-MS)and ELISA for SP-D.Lung tissues were stained by hematoxylin and eosin,and observed under microscope.Results The serum silicon levels gradually increased as the exposed dosages increased and as the exposure period prolonged in comparison with those of the control group.The serum levels of SP-D started to increase as the exposed silica dosages increased and afterward showing a lowering trend.Conclusions Serum silicon levels in exposed rats distinctively increase at the early stage when the exposure dosages are low,and it happens earlier than the changes of SP-D.Both levels vary with the exposure dosages and exposure periods.Results suggest that changes of serum silicon contents and expression of surface-active protein-D in silica exposed rats might be taken as the biomarker of early diagnosis of silicosis showing certain value and significance.更多还原