【摘要】 为确定维生素C二步发酵中巨大芽孢杆茵(伴生菌)芽孢形成对氧化葡萄酸杆菌(产酸菌)产酸的影响,本研究通过对巨大芽孢杆菌生长特性分析,选取培养12 h(未形成芽孢)和36 h(芽孢大量形成)巨大芽孢杆茵B.m 2980,检测其胞外液、胞内液以及混合液对产酸茵生成2-酮基-L-古龙酸的影响。结果表明,在未开始形成芽孢时,伴生菌胞外液、胞内液及混合液对产酸菌的生长和产酸有较低的促进作用,其中胞内液的促进能力大于胞外液;在芽孢生成后,胞外液以及混合液对产酸茵生长和产酸的促进能力显著提高。更多还原

【Abstract】 In order to determine the relationship between the spore formation of Bacillus megaterium 2980(B.m 2980,called companion bacterium) and Gluconobacter oxydans(G.o,called acid-producing strain)in vitamin C two-step fermentation,the effects of the culture supernatant,the cytosol and the mixed component solution of companion bacterium which cultured for 12 h(not forming spores) and 36 h(forming spores) on the growth of G.o and its synthesizing ability of 2-keto-L-gulonic acid(2-KGA) was studied through the analysis of B.m 2980 growth characteristics.The results showed that when the B.m 2980 spores did not form,the culture supernatant,the cytosol and mixed component solution had low effects on the growth of acid-producing strain and its 2-KGA production.The cytosol of B.m 2980 promoted the growth and2 KGA production of G.o greater than that of the culture supernatant.After the spore formation of B.m 2980,the culture supernatant and mixed component solution of B.m 2980 affected growth and 2-KGA production of G.o significantly.更多还原