【摘要】 目的:通过观察前列地尔对体外培养类缺血再灌注模型视网膜神经节细胞(RGC)自噬的影响,探讨其产生保护作用的机制。方法:以原代培养Sprague-Dawley大鼠RGC为研究对象,将培养的细胞随机分为对照组、模型组、给药组。建立类缺血再灌注损伤模型,给药组在建立模型前后加入浓度为45μg/L前列地尔。采用荧光定量PCR方法检测各组细胞中ULK1、Beclin1、Bcl-2mRNA的相对表达量。结果:①与对照组相比,ULK1mRNA、Beclin1mRNA表达均明显升高,其中模型组(P<0.01),给药组(P<0.05);而给药组ULK1mRNA、Beclin1mRNA表达较模型组明显下调(P<0.01)。②模型组与对照组相比,Bcl-2mRNA表达差异无统计学意义(P>0.05);给药组与对照组相比,Bcl-2mRNA表达升高(P<0.05),与模型组相比表达也明显升高(P<0.01)。结论:前列地尔对体外培养类缺血再灌注模型RGC可能通过抑制细胞自噬作用,进而发挥其保护细胞的作用。更多还原

【Abstract】 Objective:To evaluate the autophagy effect of alprostadil on the cultured retinal ganglion cells(RGC)in a model of ischemia-like-reperfusion in order to investigate the possible protective mechanism of it.Methods:The primary cultured RGC from the Sprague-Dawley rats were randomly divided into groups control,model and alprostadil.The model of ischemia-like-reperfusion was established.Alprostadil was applied at a concentration of 45μg/L before and after establishment of the model in group alprostadil.The relative expression of ULK1,Beclin1 and Bcl-2mRNA in the three groups was evaluated by real-time PCR. Results:As compared with group control,the expression of ULK1 mRNA and Beclin1 mRNA was increased in groups model and alprostadil(P <0.01and P <0.05;respectively),while the expression of ULK1mRNA and Beclin1mRNA was less in group alprostadil than in group model(P <0.01).There was no significant difference in the Bcl-2mRNA expression between groups control and model(P>0.05), but the expression of Bcl-2mRNA in group alprostadil was higher than that in groups control(P <0.05) or model(P <0.01).Conclusion:Alprostadil probably inhibits the autophagy effect on the cultured retinal ganglion cells in the model of ischemia-like-reperfusion then plays a role of protection.更多还原