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Label-free biomolecular imaging using scanning spectral interferometry

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【作者】王同舟；谢丽萍； Haley Huang；黎新；汪汝亮；杨光；杜亚楠；黄国亮；

【Author】 Tongzhou Wang;Liping Xie;Haley Huang;Xin Li;Ruliang Wang;Guang Yang;Yanan Du;Guoliang Huang;Department of Biomedical Engineering,Tsinghua University School of Medicine;Department of Biomedical Engineering,Johns Hopkins University;National Engineering Research Center for Beijing Biochip Technology;

【机构】 Department of Biomedical Engineering,Tsinghua University School of Medicine； Department of Biomedical Engineering,Johns Hopkins University； National Engineering Research Center for Beijing Biochip Technology；

【摘要】 This letter presents a label-free biomolecular imaging technique based on white-light interferometry and spectral detection. The method measures thickness changes caused by specific binding between biomolecules to detect the presence of certain analyte. A spectrum-shifting algorithm is developed to resolve the thickness information from the spectrum. The axial resolution of the experimental instrument can reach ～1 nm, thereby enabling detection of trace amounts(～1 ng/mm2) of proteins or DNA. This letter also presents two experiments to prove the feasibility of the method for detecting proteins and DNA without fluorescent labeling.更多还原

【Abstract】 This letter presents a label-free biomolecular imaging technique based on white-light interferometry and spectral detection. The method measures thickness changes caused by specific binding between biomolecules to detect the presence of certain analyte. A spectrum-shifting algorithm is developed to resolve the thickness information from the spectrum. The axial resolution of the experimental instrument can reach ～1 nm, thereby enabling detection of trace amounts(～1 ng/mm2) of proteins or DNA. This letter also presents two experiments to prove the feasibility of the method for detecting proteins and DNA without fluorescent labeling.更多还原

【关键词】 interferometry； letter； label； detecting； instrument； enabling； DNA； resolve； Label； thereby；

【基金】 supported by the National SupportingPlan of China(No.2012BAI23B01);the National Natural Science Foundation of China(No.81327005);the National“973”Program of China(No.2011CB707701);the National Foundation of High Technology of China(Nos.2012AA020102 and 2013AA041201);the Biomedical Detection Technology and Instrument Laboratory of Beijing Foundation

【文献出处】 Chinese Optics Letters ,中国光学快报(英文版) , 编辑部邮箱 ,2013年11期

【分类号】Q-33
【下载频次】38

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