【摘要】 为获得鲤鱼白细胞介素10(interleukin 10,IL-10)全长基因组序列,本研究利用鲤鱼IL-10全长cDNA序列(Gen-Bank登录号:JX524550),通过在两端非编码区设计引物,以提取的鲤鱼脾脏基因组为模板,使用PCR方法成功获得鲤鱼IL-10全长基因组序列。鲤鱼IL-10基因组全长2176bp,GenBank登录号为JX524551。将所获得的序列与其他物种IL-10基因组序列相比,结果发现都含有5个外显子,4个内含子,外显子在进化上相对保守,剪切位点都符合"gt......ag"规则;序列包含540bp的开放阅读框,编码179个氨基酸,有2段典型的IL-10氨基酸信号基序。更多还原

【Abstract】 The purpose of this study was to obtain the full-length sequence of common carp interleukin 10(IL-10) genomic DNA.The sequence of common carp IL-10 full-length cDNA(GenBank:JX 524550) was used.In this study,we designed two pairs of primers in its untranslated regions.Using the carp spleen genomic DNA as the template,we carried out PCR and obtained the IL-10 full-length genomic DNA sequence.The sequence was 2176 bp in length,and the GenBank accession number was JX524551.We compared the gained sequence with the other IL-10 and found their similarity of all harboring 5 exons and 4 introns.Moreover,all exons were comparatively conserved.The exons and introns splice sites were in accordance with "gt......ag" rules.Our results indicated that it was the sizes of introns that were crucial in the process of evolution.更多还原