【摘要】 目的探讨角蛋白2e在寻常型鱼鳞病(IV)皮损组织中的表达模式。方法采用S-P免疫组化方法检测10例IV患者皮损组织标本及14例正常皮肤组织标本中角蛋白2e的表达,用图像分析软件ImageProplus(IPP)判定角蛋白2e在IV患者皮损组织及正常皮肤组织中阳性表达的强弱(通过PU值反映)。结果角蛋白2e在IV皮损组织及正常皮肤组织中均有表达。在正常皮肤组织中,角蛋白2e阳性染色弥散分布于表皮角质层、颗粒层、棘层及基底层细胞;在IV皮损组织中,角蛋白2e阳性染色分布于表皮角质层、颗粒层,及少量棘层细胞,基底层细胞未见染色。染色均为胞质染色。角蛋白2e在IV皮损及正常皮肤组织的阳性染色PU值分别为0.1537±0.0097和0.2168±0.0180;角蛋白2e在IV皮损阳性染色PU值明显低于正常皮肤(t=-11.05,P<0.0001)。结论角蛋白2e在IV皮损组织中表达降低,可能与IV发病有关。更多还原

【Abstract】 Objective To investigate the expression pattern of keratin 2ein ichthyosis vulgaris ( IV ) lesions.Methods S-P immunohistochemical method was adopted to detect the expression of keratin 2ein 10IV lesion tissue samples and 14samples of normal skin tissue , both of which were from different persons , and then image analysis were performed by software ImagePro plus (IPP), which indicated the expression intensity via PU values.Results Expression of keratin 2ewas mainly localized within cyto-plasm of stratum corneum , granular layer , prickle layer and basal layer in healthy skin.Expression of keratin 2ewas mainly localized within cytoplasm of stratum corneum and granular layer in lesional skin of IV patients.The PU values of positive staining in lesional skin tissue of IV patients and healthy skin tissue were 0.153 7±0.009 7and 0.216 8±0.018 0 , respectively.The PU value of keratin 2emarkedly decreased in lesion skin tissue of IV patients compared with that of healthy skin ( t=-11.05 , P<0.0001) .Conclusion The expression of keratin 2edecreased in lesion skin tissue of IV patients , which may relate to the pathogenesis of IV.更多还原