【摘要】 目的调查深圳地区Rh阴性献血者血清学表型和不规则抗体的抗体分布情况,为安全输血提供依据。方法应用微量板盐水法筛选RhD阴性献血者、用间接抗人球蛋白试验(IAT)确认及吸收放散,不规则抗体筛选及鉴定C、c、E、e抗原表型。结果 120 566献血者初筛出456份RhD阴性(0.38%);确认为弱D/部分D 33份;吸收放散实验共筛出Del表型89份,其中Ccee 59例(66.29%)、CCee 16例(17.98%)、CcEe 7例(7.87%)、ccEe 6例(6.74%)和CCEe 1例(1.12%)。456份Rh阴性标本检出血清含有不规则抗体的标本有6例(1.32%),其中IgG类抗-D抗体4例,均有妊娠输血病史。结论深圳地区Rh阴性无偿献血者血清学具有多种表型,以dccee表型为主;有少数人产生不规则抗体,大多与有妊娠史、输血病史相关。更多还原

【Abstract】 Objective To study Rh D negative phenotypes distribution and antibody screen in blood donors in Shenzhen area.Methods RhD phenotype was initially screened by routine serological test.All of weak D or partial D phenotype was detected by indirect anti-globulin test(IAT),and Del phenotype was detected by absorption-elution test.The CcEe phenotypes and Irregular antibody were detected.Results A total of 456 RhD(-) were confirmed in 120566 donors,among 33 samples were weak D or partial D,and 89 samples were Del.The CcEe phenotypes of Del were Ccee(66.29%),CCee(17.98%),CcEe(7.87%),ccEe(6.74%) and CCEe(1.12%).A total of 6 cases were positive for irregular antibody,including 4 for anti-D and 2 for cold antibody.Conclusion The Rh D(-) phenotype of donors in shenzhen area was variety,primarily phenotype was dccee.The few donors had irregular antibodies due to pregnancies or transfusions.更多还原