【摘要】 目的通过检测乳腺癌患者外周血人乳腺珠蛋白(hMAM)水平,探讨其作为乳腺癌微转移检测指标的临床意义。方法用酶联免疫吸附实验(ELISA)法定量测定164例乳腺癌患者、40例乳腺纤维腺瘤患者及40例健康志愿者血清hMAM含量;采用SP免疫组化法对乳腺癌患者的ER、PR、Her-2、P53、Ki-67进行检测并进行分子分型。结果乳腺癌组血清hMAM水平显著高于纤乳腺纤维腺瘤组和健康对照组(P均<0.01)。乳腺癌淋巴结阳性组血清hMAM水平显著高于淋巴结阴性组;Ⅲ期患者血清hMAM水平显著高于Ⅰ+Ⅱ期患者;血清hMAM水平与肿瘤最大直径相关,随瘤体最大直径的增长,血清水平在增高,差异均有统计学意义(P=0.000)。血清hMAM水平与患者年龄、月经状况、组织学分级、ER、PR、Her-2、P53、Ki-67、分子分型等无相关性。结论 hMAM在乳腺癌患者中特异性高表达,血清hMAM水平升高可能与肿瘤的形成、发展以及转移密切相关,有可能作为判断乳腺癌外周血微转移的标志物,并在乳腺癌微转移诊断和预后判断中具有重要意义。更多还原

【Abstract】 Objective To explore the significance of human mammaglobin(hMAM) in breast cancer.Methods The hMAM level was detected in 164 patients with breast cancer,40 patients with mammary fibroadenoma and 40 healthy volunteers by enzyme-linked immunosorbent assay(ELISA).The expressions of estrogen receptor(ER),progesterone receptor(PR),human epidermal growth factor receptor 2(Her-2),protein 53(P53) and Ki-67 in 164 patients with breast cancer were detected by immunohistochemistry method.Molecular types were classified by the status of ER,PR and Her-2.Results The hMAM level in patients with breast cancer was significantly higher than that in patients with mammary fibroadenoma and that in healthy volunteers(P<0.01).The hMAM level in lymph node positive group was significantly higher than that in lymph node negative group(P=0.000),and the level of patients in stages Ⅲ were also significantly higher than that in stagesⅠ+Ⅱ(P=0.000).The hMAM level was correlated with the tumor maximum diameter(P=0.000),but it was uncorrelated with other clinical parameters(age,menstruation condition,histological grade,ER,PR,Her-2,P53,Ki-67 and molecular types).Conclusion The hMAM in peripheral blood might be specific highly expressed in patients with breast cancer.The rising of hMAM might have a close relationship with the causing,developing and metabasising of breast cancer.It could be used as a marker in detecting micrometastases and judging prognosis for breast cancer.更多还原