【摘要】 目的:探讨不同时程的慢性不可预计温和应激对大鼠海马区神经可塑性相关蛋白水平的影响。方法:24只大鼠随机分为4组,各6只:正常对照组、应激1周组、应激2周组、应激3周组,分别给予应激0、1、2、3周。采用Western blot方法检测海马脑源性神经营养因子(BDNF)、cAMP反应元件结合蛋白(CREB)、B淋巴细胞瘤-2(Bcl-2)的蛋白水平。结果:与正常对照组对比,应激1周组、应激3周组的BDNF、CREB、Bcl-2的蛋白水平较低(P<0.05),应激2周组无明显差异;应激1周组和应激3周组之间的BDNF、CREB、Bcl-2蛋白水平无明显差异。结论:海马区的神经可塑性相关蛋白BDNF、CREB、Bcl-2的水平变化可能与抑郁行为的变化存在一定的关联性。更多还原

【Abstract】 Objective: To explore the effect of different time courses of chronic unpredictable mild stress on neuroplasticity-related proteins expression in the adult rat hippocampus. Methods: Twenty-four rats were randomly divided into four groups with 6 rats in each group: the control group, rats without stress; M1 group in which rats were subjected to CUMS for 1 week; M2 group, in which rats were subjected to CUMS for 2 weeks; and M3group in which rats were subjected to CUMS for 3 weeks. The levels of brain-derived neurotrophic factor(BDNF), cAMP response element binding protein(CREB), B-cell lymphoma-2(Bcl-2) protein in the hippocampus were detected using Weston blot. Results: when compared to the expression of BDNF, CREB, and Bcl-2 proteins in the controlled group, those in the M1 and M3 groups were significantly decreased(P<0.05), and there was no different changes in those protein in the M2 group. The amounts of BDNF, CREB, and Bcl-2 protein showed no significant difference between the M1 group and M3 group. Conclusion: Different time courses of CUMS might affect the expression of proteins implicated in neuroplasticity, providing insight into the role of neuroplasticityrelated proteins in the depression.更多还原