【摘要】 建立了气相色谱串联质谱(G C-M S)准确、快速测定香精香料中仲丁醇、富马酸二甲酯、咪唑和芝麻酚的分析方法。样品经甲醇超声提取15min后,通过Agilent DB-WAX(0.25mm×30m×0.25Lm)色谱柱分离,质谱进行定性,外标法对于4种目标物质进行定量分析。实验结果表明,在0.5—20mg/L的范围内,4种物质具有较好的线性,各待测物质的线性相关系数均大于0.9990,检出限为0.02—0.12mg/L,在1,5mg/L和10mg/L 3个加标水平条件下,4种禁限用物质的平均回收率为88.0%—113.1%,相对标准偏差为0.32%—6.53%。该方法前处理简单,灵敏度高,线性相关性好,重复性佳,可以满足香精香料中4种物质的同时检测。更多还原

【Abstract】 A gas chromatography coupled with mass spectrometry as detector(GC-MS) was established for the separation and determination of 4 substances(s-butanol, dimethyl fumarate, imidazole,sesamol) in flavor and fragrant samples.After extracted by HPLC methanol,the target compounds were separated on an Agilent DB-WAX capillary column(0.25mm×30m×0.25Lm). Selected ion monitoring(SIM) mode of GC-MS was used in quantitation.It’s exhibited that there was a good linear relationship(>0.9990) ranged from 0.5 to 25mg/L and the limits of detection was in range of 0.02—0.12mg/L.The average recoveries at three levels were in the range of 88.0%—113.1% with the relative standard deviations(RSD)ranged from 0.32% to 6.53%.This simple method can be used to determine 4 substances in flavor and fragrant samples simultaneously.更多还原