【摘要】 建立了超高效液相色谱-二极管阵列检测器(UPLC-PDA)同时分离测定香精香料中糖精钠,芝麻酚,酸性橙Ⅱ,碱性嫩黄O的方法。样品经过10%的甲醇水溶液(含1%氨水)提取后,以Waters BEHC18(1.7μm,2.1mm×50mm)为色谱柱,5mmol/L乙酸铵(含0.1%乙酸)和乙腈(ACN)为流动相,200—500nm波长范围内进行扫描检测。结果表明,在5min以内4种目标物质得到了较好的分离,在0.5—20mg/L范围内具有较好的线性(r>0.9950),检出限为0.08—0.61mg/L,3种浓度水平的加标回收率保持在85.3%—110.3%之间,相对标准偏差在6%以内。该方法操作简单,灵敏度高,可以满足香精香料中4种物质的同时检测。更多还原

【Abstract】 An ultra high performance liquid chromatography method coupled with Photo Diode Array Detector(PDA) as detector was established for the separation and determination of 4 substances(sodium saccharin,sesamol,acid orange Ⅱ,auramine O) in flavor and fragrant samples.After extracted by 10% methanol/water solution(containing 1% ammonia V/V),the target compounds were separated on a Waters BEH C18 column(1.7μm,2.1mm×50mm) using HPLC acetonitrile and 5 mmol/L ammonium acetate aqueous solution(with 0.1%(V/V)) acetic acid) as mobile phases,and detected by a diode array detector scanned from 200 nm to 500 nm.It’s exhibited that there was a good linear relationship(r>0.9950) ranged from 0.5 to 20mg/L and the limits of detection was in range of 0.08—0.61mg/L.The average recoveries at three levels were in the range of 85.3%—110.3% with the relative standard deviations(RSD)less than 6%.This simple method can be used to determine 4 substances in flavor and fragrant samples simultaneously.更多还原