【摘要】 目的:在我院建立简易的幽门螺杆菌培养方法。方法:胃镜下取患者胃黏膜,尿素酶试剂盒检测幽门螺杆菌为阳性者进行培养,在微需氧环境中培养5d,取原代传代。原代菌株用尿素酶法、过氧化氢酶法、氧化酶法和革兰染色鉴定,同时符合者为幽门螺杆菌。结果:53例胃黏膜标本培养,45例培养出幽门螺杆菌,成功率为84.91%,通过以上方法鉴定符合幽门螺杆菌标准。结论:建立了简单有效的培养幽门螺杆菌的方法。更多还原

【Abstract】 Objective:To establish a simple method of culturing Helicobacter pylori(H pylori) in our hospital.Methods:The gastric mucosa taken under gastroscope and tested positive by urease test were cultured in microaerophilic environment for 5 days,and the primary bacterias were passaged.The primay bacterias were identified by urease test,catalase test,oxidase test and Gram staining.All the results accorded with H pylori were considered successful.Results:Of the culture of the gastric mucosa in 53 cases,45 specimen were successful to develop the standard H pylori determined by the aboved test methods,which was of the success rate of 84.91%.Conclusion:Simple and effective method to culture helicobacter pylori was constructed.更多还原