【摘要】 以科尔沁和浑善达克沙地的6个天然白榆种群为研究对象,采用RAPD分子标记技术,从35条引物中筛选出扩增多态性较多且扩增产物稳定的17条10bp引物。对6个白榆种群的180个叶片样品的基因组DNA进行扩增,共检测到181个位点,多态位点有96个,多态位点百分比为52.89%;不同种群内的遗传多样性水平差异不大,Nei’s多样性指数(h)和Shannon’s多样性指数(I)为0.2611和0.3814,遗传多样性主要存在于种群内;群间的遗传分化系数Gst为0.3768,种群内的遗传变异所占比例为62.32%;6个种群的遗传距离显示,科尔沁沙地和浑善达克沙地白榆各种群间的遗传距离不明显。更多还原

【Abstract】 The genetic diversity and genetic structure of six Ulmus pumila populations in Horqin Sand Land and Otingdag Sand Land were assessed by using RAPD markers.17 primers were chosen from 35 kinds of 10bp random primers,which could produce polymorphic,clear and stable bands.From 180 individuals of 6 populations,17 primers yielded 181 clearly identifiable bands,and among them 96 polymorphic bands were polymorphic.As analyzed by POPGENE1.32,the percentage of polymorphic loci(PPB) was 52.89%;Nei’s index and Shannon’s index of the gene diversity were 0.2611 and 0.3814 in special.The genetic differentiation was Gst =0.3768.The genetic variation among populations was37.68%,and within population was 62.32%.The genetic diversities in 6 populations were similar,Between two sandland genetic distance is not obvious.更多还原