【摘要】 在生物技术和生命科学等领域常依赖抗体的特异识别作用来实现目标蛋白质的分离、分析和检测。以蛋白质为模板的分子印迹聚合物有望取代这些昂贵的抗体,但交联聚合物网络中大分子的传质限制是蛋白质分子印迹研究中面临的一个重要问题。纳米结构的蛋白质印迹材料具有较高的表面积与体积比及较浅的印迹位点,很大程度上可克服这一问题。本文详细介绍各种纳米蛋白质印迹聚合物的制备方法,分析各自的优势和不足,并简要介绍其应用。更多还原

【Abstract】 In the areas such as biotechnologies and life science,separation,analysis and detection of a target protein is achieved commonly relying on antibody-based specific interactions.Molecularly imprinted polymers against proteins are regarded as the most potential substitutes for the costly antibodies.However,successful imprinting of a protein is faced with some problems,one of which is the limited macromolecule diffusion throughout the cross-linked protein-imprinted polymer networks.Nanostructured protein-imprinted polymers can overcome this problem to a great extent,with relatively high ratio of surface area to volume and easily accessed imprinting sites.Herein,we review various approaches reported previously for constructing the nanosized protein-imprinted materials,particularly addressing the advantages and shortcomings associated with each method.The potential applications of these protein-imprinted materials are also described.更多还原