【摘要】 目的建立MagA转基因小鼠,为活体MRI成像系统提供重要的实验动物模型。方法采用显微注射法,将MagA基因导入小鼠受精卵,再培育成转基因小鼠并繁殖传代。利用PCR方法鉴定转基因首建鼠及其子代基因型。结果酶切和DNA测序分析证实MagA基因准确克隆表达载体设计位点,目的基因序列与GenBank中MagA序列完全一致。通过PCR分析,进行转基因整合检测。目前转基因小鼠已稳定传代至第5代。结论 MagA基因在转基因小鼠中整合,成功建立了MagA转基因小鼠。MagA转基因小鼠将成为MRI影像系统的重要实验动物模型。更多还原

【Abstract】 Objective To establish MagA transgenic mice models for in vivo MRI imaging studies to provide important experimental animal models. Methods Microinjection was used with MagA genes into mice fertilized eggs, and then develop into transgenic mice. The transgenic mice were genotype by PCR. Results Enzyme digestion and DNA sequencing analysis confirmed that MagA gene cloning expression vector design site accurately, the purpose gene sequences are in complete accord with MagA sequences in GenBank. Detection of genetically modified integration by PCR analysis. The transgenic mice has stable to extend to the fifth generation. Conclusion The resutlts of PCR analysis of MagA genes in transgenic mice expression, MagA transgenic mice established successfully. MagA transgenic mice for in vivo MRI imaging system will become an important experimental animal model.更多还原