【摘要】 目的研究茶多酚表没食子儿茶素没食子酸酯(EGCG)对体外培养鼻咽癌细胞的抑制作用。方法选用HNE1细胞系,采用四甲基偶氮唑盐微量酶反应比色法(MTT法)和台盼蓝染色法检测不同浓度EGCG分别在给药不同时间后对HNE1细胞体外增殖的抑制作用。结果 MTT法和台盼蓝法都发现EGCG对HNE1细胞体外增殖的抑制作用有浓度-时效关系。这两种方法都适用于检测EGCG对HNE1细胞体外增殖的抑制作用。结论 EGCG也许能用于鼻咽癌的预防和治疗。更多还原

【Abstract】 Objective To investigated green tea polyphenol epigallocatechin-3-gallate(EGCG) against nasopharyngeal car- cinoma in vitro. Methods HNE1 cell line was used. EGCG in various concentrations was applied to HNE1 growth. The MTT stain method and trypan blue method were used as markers to evaluate the effect of EGCG on HNE1. Results EGCG inhibits HNE1 cell growth in a dose-dependent and time-dependent manner according to MTT and trypan blue methods. These two methods can be used to detect EGCG against HNE1 cell in vitro. Conclusion EGCG can be used for the prevention and treatment of nasopharyngeal car- cinoma.更多还原