【摘要】 目的研究充血性心力衰竭大鼠的大脑海马区TGF-β1变化。方法将85只成年雄性SD大鼠随机分为正常组5只、假手术组40只、模型组40只,假手术组和模型组观察时间点为术后1、2、4、8周。采用腹主动脉部分缩窄术,建立大鼠慢性充血性心力衰竭模型。应用超声心动图动态监测左室舒张末期内径(LVEDd),舒张末期室间隔厚度(IVSd)和左室后壁厚度(LVPWd);解剖心脏,计算心肌质量指数[心质量/体质量(HW/BW)和左室质量/体质量(LVW/BW)],免疫组织化学染色法检测大脑海马区TGF-β1表达变化。结果模型组LVEDd、IVSd、LVPWd高于正常组和假手术组,且呈时间依赖性上升趋势(P<0.01)。模型组心肌质量指数与正常组和假手术组比较,差异有统计学意义(P<0.01)。模型组TGF-β1表达量明显高于假手术组。结论压力超负荷性心力衰竭发生过程中大脑海马区TGF-β1表达量明显增高。更多还原

【Abstract】 Objective To investigate the expression of transforming growth factor β1(TGF-β1) of hippocampus in rats with heart failure. Methods 8 weeks after establishment of heart failure model, the femal SD rats were randomly assigned to : AMI con- trol group and AMI + FVgroup, and sham operated group was selected randomly as non infarction control. After 8 weeks, cardiac function, hemodynamics, ventricular remodeling parameters, TGF-β protein expression in hippocampus area were measured. Results As compared with sham-operated group, LV end-diastolic dimension(LVEDD), LV end-diastolic volume(LVEDV), E / A ratio, LV end diastolic pressure(LV-EDP), relative weight(LVRW), right ventricular relative weight(RVRW); TGF-β pro- tein in hippocampus were all significantly increased in heart failure group(P<0.05). Conclusion The proteins of TGF-β1 in hip- pocampus is related to the pathophysiologic processes after heart failure.更多还原