【摘要】 目的探讨人参皂苷Rb1(ginsenoside Rb1,Gs-Rb1)减轻阿霉素所致的慢性心力衰竭(CHF)效应与改善葡萄糖转运载体-4(glucose transporter-4,GLUT-4)的关系。方法阿霉素诱导CHF大鼠被随机分为对照组(n=10)、阿霉素组(n=15)和Gs-Rb1组[70 mg/(kg.d),n=17],同时培养的乳鼠心肌细胞随机分为对照组、阿霉素组(1μmol/L)和Gs-Rb1组(200μmol/L)。干预完毕后,检测心脏超声、GLUT-4蛋白和mRNA水平。结果 (1)与阿霉素组相比,Gs-Rb1组的左室射血分数显著提高(P<0.05);(2)在体内研究中,Gs-Rb1组GLUT-4蛋白和mRNA水平均显著高于阿霉素组(P<0.05),但显著低于对照组(P<0.05);(3)在体外研究中,与阿霉素组相比,Gs-Rb1组GLUT-4蛋白水平和mRNA水平显著提高(P<0.05),但均显著低于对照组(P<0.05);(4)阿霉素组心肌细胞膜GLUT-4蛋白水平显著低于对照组(P<0.05),而Gs-Rb1组心肌细胞膜GLUT-4蛋白显著高于阿霉素组(P<0.05)。结论在阿霉素促发CHF进程中,GLUT-4的表达和移位被抑制可能起着一定作用;Gs-Rb1改善阿霉素CHF效应与促进GLUT-4的表达和移位有关。更多还原

【Abstract】 Objective On the basis of adriamycin-induced chronic heart failure(CHF),to elucidate the effect of ginsenosides-Rb1(Gs-Rb1) on improving cardiac function via affecting glucose transporter-4(GLUT-4) in vivo and in vitro.Methods CHF rats were induced with adriamycin and randomly divided into control group(n=10),adriamycin group(n=15) and Gs-Rb1 group [70 mg/(kg·d),n=17].The neonatal rat cardiomyocytes were randomly divided into control group,adriamycin group(1 μmol/L) and Gs-Rb1 group(200 μmol/L).At the 4th week,the left ventricular ejection fraction(LVEF) was analyzed by echoeardiography,as the expression of GLUT-4 were assessed by Western blot and RT-PCR.Results Compared to adriamycin group,Gs-Rb1 significantly improved LVEF(P=0.015).Both protein and mRNA of GLUT-4 were significantly up-regulated in Gs-Rb1 group than those in adriamycin group,but significantly lower than those in control group in vivo;so was in vitro.The expression of GLUT-4 protein in the membrane of cardiomyocytes was significantly lower in adriamycin group than those in Gs-Rb1 group and control group.Conclusion The inhibition of both expression and transformation of GLUT-4 involve in the pathogenesis of adriamycin-induced CHF,which could be partially reversed by Gs-Rb1.更多还原