【摘要】 目的探讨细胞因子信号传导抑制因子-1(SOCS1)基因腺病毒转染对未成熟的树突状细胞(DC)的成熟及细胞因子分泌功能的影响。方法构建SOCS1腺病毒并PCR鉴定,感染体外分离、培养的小鼠骨髓DC(阳性转染组),Western blot法检测SOCS1的表达,以阴性转染组(转染Ad-GFP)及空白对照组(未经病毒转染)为对照,采用脂多糖(LPS)刺激诱导各组DC成熟,通过流式细胞术测定LPS刺激前后各组DC表面CD40及CD80的表达水平,ELISA法检测细胞因子白细胞介素(IL)-10及IL-12的分泌水平。结果成功构建并鉴定转染腺病毒,感染DC后,阳性转染组SOCS1蛋白表达水平较阴性转染组及空白对照组显著增高。LPS刺激后,阳性转染组CD40及CD80的表达较阴性转染组及空白对照组显著降低(P<0.05),IL-12分泌水平明显较低(P<0.05),而IL-10分泌水平显著增高(P<0.05)。结论通过腺病毒转染使SOCS1在DC中过度表达,不仅能有效抑制DC的成熟,还能抑制LPS介导的DC分泌细胞因子IL-12,并诱导抑制性细胞因子IL-10的分泌。更多还原

【Abstract】 Objective To investigate the effect of suppressor of cytokine signaling-1(SOCS1) by transfection of adenovirus vectors on the phenotype and immunologic function of dendritic cells.Methods The adenovirus vectors containing SOCS1 was constructed and confirmed by PCR.The phenotype CD40 and CD80,and cytokine secretion of denderitic cells was measured after LPS stimulation by flow cytometry and ELISA methods.Results The expression of SOCS1 in dendritic cells transfected by adenovirus vectors(DC-SOCS1) was efficient.DC-SOCS1 showed significantly lower expressions of CD40 and CD80,and reduced IL-12 despite of the stimulation of LPS(P<0.05).Furthermore,significantly higher IL-10 was found in the DC-SOCS1 compared to control group(P<0.05).Conclusion SOCS1 expression could block maturation and secretion of inflammatory cytokine IL-12,and promote production of inhibitory cytokine IL-10 induced by LPS in DC.更多还原