【摘要】 为了研究马尾松(Pinus massoniana Lamb.)4CL基因的遗传进化及系统发育情况,根据NCBI上已登录的4CL基因的序列,比对分析,利用同源序列设计引物,以马尾松基因组DNA为模板,扩增核酸序列,与其他21种松属植物4CL基因进行SNP对比分析。结果表明:扩增出474bp核酸序列,松属植物4CL基因共存在28个SNP位点,总核苷酸多样性θw=0.034 83、πT=0.036 21,具较高的多态性。系统进化树聚类结果表明,22个松属植物均集中在第一、二大类,马尾松、火炬松、班克松和小干松聚为第一大类中的第二亚类,亲缘关系较近。更多还原

【Abstract】 To explore the genetic evolution and phylogenetic of 4 CL gene family fromP.massoniana, the 4CL gene in P.massoniana was cloned,during which,the consensus sequences of previous publish in NCBI were used to design the primers and PCR was performed on genomic DNA of P.massoniana.The results showed that there were 28 single nucleotide polymorphisms(SNP)marker between the 22 4CL gene sequences of genus Pinus.The average nucleotide diversity for the sequenced regions was calculated to beθw=0.034 83andπT =0.036 21,with higher polymorphism.Alignment of sequences of 4 CL gene with MEGA 5.2 indicated that 22 genus Pinus were clustered together to two major groups,showed a low genetic divergence among the populations of 22 genus Pinus in their evolution process,but there was a significant interspecific difference. The closer relationship was for P. massoniana,P.taeda, P.banksianaand P.contorta.The evolution of 4 CL gene in the process of plant evolution were relatively conservative,because of extensive distribution of pine,so polymorphism of 4CL gene was high,the divergence should be fully considered in the 4 CLgene research.更多还原