【摘要】 以实验室构建的产SOD重组菌为出发菌株,研究其摇瓶发酵工艺条件以提高表达水平,进而在5 L高密度发酵.以确定接种后诱导时间(16 h)、诱导剂浓度(2%)、温度(30℃)、初始pH值(6.2)和诱导时间72 h为条件,摇瓶水平酶活比初始时提高了64%;经5 L罐发酵实验,酶活达到40 864 U·mL-1,是摇瓶的86倍.更多还原

【Abstract】 In this article,study fermentation conditions of the recombinant strains in shake culture,aiming at improving the protein expression and fermenting in 5 L fermentation tank. The result indicates that the suitable fermentation conditions for the recombinant cell are as below: the temperature of cell growth and protein expression is 30 ℃,the methanol feeding is 2%( volume ratio of the medium)per day,the initial pH is 6. 2,the induction time is 72 h. According to these conditions,the enzyme activity of shake culture is increased by 64% comparing with before optimization. In 5 L fermentation cultivate,the enzyme activity reaches 40 864 U·mL-1,86 times of the shake culture.更多还原