【摘要】 克隆与辐射抗性相关但功能未知的新基因DR₂566,并应用生物信息学初步探讨其功能。应用PCR技术从耐辐射奇球菌基因组中扩增DR₂566基因全长ORF,选用pGEM-T载体进行TA克隆,通过限制性酶切及测序进行鉴定。应用生物信息学初步分析其染色体定位、蛋白序列、结构域及功能。成功克隆了耐辐射球菌未知功能基因DR₂566,生物信息学分析显示此基因全长为507 bp,编码168个氨基酸,其编码蛋白的理论分子量为19.136KDa,三级结构预测显示DR₂566蛋白质具有短修复内切酶性质。成功克隆了耐辐射奇球菌基因DR₂566全长ORF,生物信息学预测DR₂566蛋白可能具有错配修复作用。更多还原

【Abstract】 The objective of this study was to clone DR₂566 and to investigate its function preliminary using bio-informatics.Full-length open reading frame（ORF） of DR₂566 gene was amplified by PCR,and pGEM-T was chosen as a vector for gene TA cloning.After ligation,restriction endonuclease analysis and sequencing were carried out for identification.On the other hand,bio-informatics was used to analyze the localization of the target gene on the chromosome,speculate protein sequence,domain and function.DR₂566 gene was cloned successfully.Bio-informatic analysis results show that the target gene,with 507 bp full-length,encodes a protein with 168 amino acids and the theoretical molecular mass of this protein is about 19.136 KDa.Tertiary structure predicting indicates DR₂566 protein is a kind of endonuclease that can repair short DNA damage fragment.Full-length ORF of DR₂566 in D.Radiodurans was cloned successfully.Bio-informatic analysis indicated the DR₂566 protein might be involved in mismatch repairing function in D.Radiodurans.更多还原