【摘要】 在盐酸介质中,基于痕量头孢氨苄(CPX)对β-环糊精(β-CD)增敏KBrO3-KBr氧化亚甲蓝(MB)体系的荧光具有增强作用,提出了一种新的测定头孢氨苄荧光分析方法。该体系最大激发波长为λex=660 nm,最大发射波长为λem=678 nm,线性范围为0.001～3.5 mg/L,检出限为0.0002 mg/L,相对标准偏差为1.3%。方法可作为痕量头孢氨苄的分析方法。更多还原

【Abstract】 A new spectrofluorimetric method with very high sensitivity and good selectivity for determining trace amount of cephalexin(CPX) has been proposed.The method is based on the enhancement of CPX on the fluorescence intensity of the product yielded in the redox reaction between Br2 and MB in hydrochloric acidic medium,in which β-CD acts as a sensitizing effect reagent.Its maximum excitation wavelength is 660 nm,and the maximum emission wavelength is 678 nm.The linear range,detection limit and relative standard deviation are 0.001~3.5 mg/L,0.0002 mg/L and 1.3%,respectively.The method was successfully applied to the assay of CPX in pharmaceutical formulations with satisfactory results.更多还原